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  - WN.COM - Science - 15/Jul 05:22

Ancestry Bias Could Cause CRISPR Screens to Veer Off-target

CRISPR-Cas9 enables researchers to make precise and targeted edits in the genome to determine gene function. For this, scientists use guide RNAs, which are short stretches of RNA sequences that lead the Cas9 enzyme to its target region of the genome. The enzyme causes double-stranded DNA breaks, which consequently turns off gene function when a cell cannot correctly repair the break. In 2017, Jesse Boehm, a genomics scientist at the Broad Institute of MIT and Harvard, and his team had used multiple guide RNAs to perform a genome-wide screen in cancer cells lines from 1,000s of patients.1 They used the CRISPR-Cas9 system to target each gene and identified many “cancer dependencies”—genes...

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